免疫浸润:生信论文44乳腺癌 引文写作

原创
2020/08/25 07:17
阅读数 232

生信论文的套路

  1. ONCOMINE从全景、亚型两个维度做表达差异分析;

  2. 临床标本从蛋白水平确认(或HPA数据库),很重要;

  3. Kaplan-Meier Plotter从临床意义的角度阐明其重要性;

  4. cBio-portal数据库做基因组学的分析(机制一);

  5. STRING互作和GO/KEGG分析探讨可能的信号通路(机制二);

  6. TISIDB/TIMER分析肿瘤免疫特征(机制三)。


引文的基本内容:介绍背景(一段或两段)、提出问题(一段),解决问题(一段或2段)。一般引文介绍3~5段。


  1. 疾病或基因的背景介绍;

  2. 前人研究的成果、现实情况,存在的问题;

  3. 阐明研究原因或意义。介绍研究的性质、范畴及其重要性,突出研究目的和待解决的科学问题;

  4. 研究方法,新发现和意义。


乳腺癌是女性第一大癌,肺癌是男性第一大癌,是我们生信分析的首选。

生信论文的背景介绍有两种通用的方式。整体而言,第二种思路的说服力稍弱。


第一种是从疾病入手,即从待研究的肿瘤说起,再介绍该肿瘤的发病率、死亡率、预后和治疗情况,从而提出科学问题,引出待研究的基因;接着介绍基因的基本信息,阐释其功能,归纳式提出该基因在肿瘤中的潜在作用。


第二种是从基因入手,即先介绍基因的基本信息,阐释其功能,在阐释说明该基因在某肿瘤中的作用尚不清楚,从而提出科学问题;接着从待研究的肿瘤说起,介绍该肿瘤的发病率、死亡率、预后和治疗情况,然后解释说该基因可能在该肿瘤中具有重要作用。


第一段,从JAK1基因的功能写起,介绍其作用机制(绿色部分),然后阐释其与肿瘤的关系,最后描述JAK1在免疫微环境的作用,为引出其在乳腺癌中的作用做好铺垫。


Janus kinases are a family of non-receptor tyrosine kinases which are involved in autoimmune diseases and malignancies [1, 2]. Januskinase-1 (JAK1) is one of the Janus kinase family members. JAK1 is essentialfor IL-6-class inflammatory cytokine signaling, plays a critical role in metastatic cancer progression, and mediates the persistent oncogenic activation of STAT3 in mammary cancer cells that are driven by ERBB2 receptor tyrosine kinase signaling [3]. JAK1-deficient cell lines were found to be more tumorigenic than wild-type cells [4]. The evidence indicates that JAK1 works as either an oncogene or a tumor suppressor under certain conditions or cell contents [5].Clinical trials testing of JAK1 inhibitors in advanced solid tumors, including breast cancer, are under way [6]. JAK1 is alsoexpressed in diverse cell types, including immune cells. A recent study hasshown that  JAK/STAT  inhibition acts  on  the tumor microenvironment  to  increase production  of pro-tumorigenic inflammatory factors in breast cancer patients, which promotes therapeutic resistance[7]. Whether JAK1 levels in breast cancer tissues are associated with tumor immune infiltrates and clinical outcomes has not been evaluated.

 

第二段介绍乳腺癌的流行病学,诊断、预后和生存现状,引出乳腺癌预后指标的话题。

 

Breast cancer mortality remains the second leading cause of female cancer-related deaths worldwide [8]. Extensive efforts are underway to develop molecular signatures and targeted therapies for specific subsets of breast cancer patients [9]. Inrecent decades, the prognostic and predictive value of mRNA expression has becomemore  attractive. Studies of the transcriptome,including mRNA levels, in primary breast tumors have been useful for predicting intrinsic subtypes, tumor grade, drug responsiveness, and risk of recurrence, (为相关性分析做铺垫) each of whichcan be used as a prognostic tool [10–12].

 

第三段解决问题。经典三句式,前两句总结分析内容 (差异分析、生存分析、相关性分析和免疫浸润分析),最后根据结果给出结论。


Here, we evaluated the association between tumor JAK1 mRNA levels and breast cancer patients’ prognosis in public databases such as the Kaplan-Meier plotter, PrognoScan database, and the Human Protein Atlas database. Moreover, we also investigated the correlation of JAK1 mRNA levels with clinicopathological characteristics and tumor-infiltrating immune cells of breast cancer patients. Our findings shed light on the important role of JAK1 in breast cancer as well as providing a potential relationship and an underlying mechanism between JAK1 and tumor-immune interactions.


本文分享自微信公众号 - 芒果先生聊生信(from2019to2020)。
如有侵权,请联系 support@oschina.cn 删除。
本文参与“OSC源创计划”,欢迎正在阅读的你也加入,一起分享。

展开阅读全文
打赏
0
0 收藏
分享
加载中
更多评论
打赏
0 评论
0 收藏
0
分享
返回顶部
顶部